LymphedemaV1, Main, Exploration, bibRecord, 007164

PERSISTENCE OF BRUGIA MALAYI DNA IN VECTOR AND NON-VECTOR MOSQUITOES: IMPLICATIONS FOR XENOMONITORING AND TRANSMISSION MONITORING OF LYMPHATIC FILARIASIS

Identifieur interne : 007164 ( Main/Exploration ); précédent : 007163; suivant : 007165

PERSISTENCE OF BRUGIA MALAYI DNA IN VECTOR AND NON-VECTOR MOSQUITOES: IMPLICATIONS FOR XENOMONITORING AND TRANSMISSION MONITORING OF LYMPHATIC FILARIASIS

Auteurs : Peter Fischer ; Sara M. Erickson ; Kerstin Fischer ; Jeremy F. Fuchs ; Ramakrishna U. Rao ; Bruce M. Christensen ; Gary J. Weil

Source :

The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 2007.

RBID : PMC:2196403

Descripteurs français

KwdFr :
- ADN des helminthes (analyse), Aedes (parasitologie), Animaux, Brugia malayi (génétique), Brugia malayi (isolement et purification), Culex (parasitologie), Filariose lymphatique (diagnostic), Filariose lymphatique (transmission), Humains, Réaction de polymérisation en chaîne (), Sensibilité et spécificité.
MESH :
- analyse : ADN des helminthes.
- diagnostic : Filariose lymphatique.
- génétique : Brugia malayi.
- isolement et purification : Brugia malayi.
- parasitologie : Aedes, Culex.
- Animaux, Filariose lymphatique, Humains, Réaction de polymérisation en chaîne, Sensibilité et spécificité.

English descriptors

KwdEn :
- Aedes (parasitology), Animals, Brugia malayi (genetics), Brugia malayi (isolation & purification), Culex (parasitology), DNA, Helminth (analysis), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (transmission), Humans, Polymerase Chain Reaction (methods), Sensitivity and Specificity.
MESH :
- chemical , analysis : DNA, Helminth.
- diagnosis : Elephantiasis, Filarial.
- genetics : Brugia malayi.
- isolation & purification : Brugia malayi.
- methods : Polymerase Chain Reaction.
- parasitology : Aedes, Culex.
- transmission : Elephantiasis, Filarial.
- Animals, Humans, Sensitivity and Specificity.

Abstract

Xenomonitoring (detection of filarial larvae or their DNA in mosquitoes) is a sensitive marker for assessing the endemicity of filariasis and a useful tool for evaluating elimination programs. To examine the fate of microfilariae (mf) and filarial DNA in vector competent and non-competent mosquito strains, we compared the detection of Brugia malayi parasites by dissection and by quantitative real-time polymerase chain reaction (PCR) in three different mosquito strains. We conclude that PCR is much more sensitive than dissection for detecting filarial larvae, especially their remnants in mosquitoes. However, parasite DNA can be detected in both vector and non-vector mosquitoes for two weeks or longer after they ingest mf-positive blood. Thus, although xenomonitoring with vector and non-vector mosquito species may be a sensitive method for indirectly detecting filarial parasites in human populations, positive test results for parasite DNA in mosquitoes do not necessarily prove that transmission is ongoing in the study area.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2196403

PubMed: 17360875
PubMed Central: 2196403

Affiliations:

Le document en format XML

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 DNA IN VECTOR AND NON-VECTOR MOSQUITOES: IMPLICATIONS FOR XENOMONITORING AND TRANSMISSION MONITORING OF LYMPHATIC FILARIASIS</title>
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<term>Culex (parasitology)</term>
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<term>Elephantiasis, Filarial (diagnosis)</term>
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<front><div type="abstract" xml:lang="en"><p id="P1">Xenomonitoring (detection of filarial larvae or their DNA in mosquitoes) is a sensitive marker for assessing the endemicity of filariasis and a useful tool for evaluating elimination programs. To examine the fate of microfilariae (mf) and filarial DNA in vector competent and non-competent mosquito strains, we compared the detection of <italic>Brugia malayi</italic>
 parasites by dissection and by quantitative real-time polymerase chain reaction (PCR) in three different mosquito strains. We conclude that PCR is much more sensitive than dissection for detecting filarial larvae, especially their remnants in mosquitoes. However, parasite DNA can be detected in both vector and non-vector mosquitoes for two weeks or longer after they ingest mf-positive blood. Thus, although xenomonitoring with vector and non-vector mosquito species may be a sensitive method for indirectly detecting filarial parasites in human populations, positive test results for parasite DNA in mosquitoes do not necessarily prove that transmission is ongoing in the study area.</p>
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Serveur d'exploration sur le lymphœdème

PERSISTENCE OF BRUGIA MALAYI DNA IN VECTOR AND NON-VECTOR MOSQUITOES: IMPLICATIONS FOR XENOMONITORING AND TRANSMISSION MONITORING OF LYMPHATIC FILARIASIS

PERSISTENCE OF BRUGIA MALAYI DNA IN VECTOR AND NON-VECTOR MOSQUITOES: IMPLICATIONS FOR XENOMONITORING AND TRANSMISSION MONITORING OF LYMPHATIC FILARIASIS

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
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